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Transgenic Production
Facility
The
Transgenic Production Facility (TPF) is an integral part of the
Wales Gene Park providing a resource to scientists who wish to create
novel transgenic and knockout strains of mice to advance their study
of genetics. In addition, the facility offers strain rederivation,
IVF and gamete cryopreservation services. Training for all of the
above services is also offered. The TPF provides a high quality
service to support both local scientists and those working further
afield. The TPF is staffed by highly experienced technicians.
The
TPF is housed within a state-of-the-art barried unit, and therefore
all services carried out result in mice of an SPF health status.
Service
details:
| Pronuclear Microinjection |
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- To produce transgenic mice
- DNA construct provided by researcher
- 200+ embryos injected
- Tail biopsies sent to researcher
- Identified Founder transgenics shipped
to client
- All sizes of construct catered for,
including YACs and BACs
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COST - from £2000
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| Blastocyst Microinjection |
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- To produce mice with specific genes knocked out, or to
study development of mutant cells in chimaeric embryos
- ES Cells provided by researcher
- 60+ blastocysts injected
- Chimaeras shipped to client
- Germline transmission established where requested
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COST
- from £2000 |
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| Rederivation |
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- To improve the health status or as
an assisted reproductive technique for problem breeders
- Service tailored to suit your needs
- Send us cryopreserved or fresh embryos,
or core stock of mice
- SPF mice shipped to the researcher
following a clear health screen
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COST
- from £600 |
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| In Vitro Fertilisation |
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- For rederivation or assisted reproduction to produce large
numbers of offspring
- Send us fresh or frozen sperm, or male mice
- A wide range of background strains can be used as embryo
donors, to suit the researcher's needs
- All animals produced will be SPF
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COST
- from £1000 |
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| Cryopreservation |
- Can be used to provide a safeguard against unexpected
loss of colonies, to minimise genetic drift over long periods
of time and to preserve valuable models where there is no
present need to maintain the colony.
- Embryos cryopreserved at 8-cell stage
- Sperm cryopreserved from stud males, non breeding or geriatric
males, or even recently deceased animals
- Oocytes cryopreserved for use in IVF
- Ovaries cryopreserved as an assisted reproductive technique
for non-breeding females or as strain storage
- Viability testing included for all strains cryopreserved
COST - Subject to requirements
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| Training |
- The TPF also offers training in all off the aforementioned
services, at our facility in Cardiff or at your own laboratory.
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Contact:
For detailed service
details and application forms please contact asbyom@cf.ac.uk
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